Purified Rabbit anti-BRCA1 (pS1524) Antibody

SKU: DL92148A

Size 1: 100 ul

Synonyms : RNF53; Breast cancer type 1 susceptibility protein; RING finger protein 53

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Rabbit polyclonal antibody to BRCA1 (pS1524)

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BRCA1 (pS1524). The exact sequence is proprietary.

WB description: Western blot analysis of BRCA1 (pS1524) expression in A431 (A), NIH3T3 (B), PC12 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of BRCA1 (pS1524) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)

Gene ID(Human): 672

Gene ID (Mouse): 12189

Gene ID(Rat): 497672

SwissProt (Human): P38398

SwissProt (Mouse): P48754

SwissProt (Rat): O54952

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.