Purified Rabbit anti-BAX Antibody

SKU: DL90092A

Size 1: 100 ul

Synonyms : BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R, B, P

Applications: ELISA, WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to BAX

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BAX. The exact sequence is proprietary.

WB description: Western blot analysis of Calmodulin expression in HeLa (A), MCF7 (B), Ramos (C), NIH3T3 (D) whole cell lysates.

IHC description: Immunohistochemical analysis of Calmodulin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Calmodulin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Gene ID(Human): 801; 805; 808

Gene ID (Mouse): 12313; 12314; 12315

Gene ID(Rat): 24242; 24244; 50663

SwissProt (Human): P62158

SwissProt (Mouse): P62204

SwissProt (Rat): P62161

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.