Purified Rabbit anti-AMPK beta 2 Antibody

SKU: DL97044A

Size 1: 100 ul

Synonyms : 5'-AMP-activated protein kinase subunit beta-2; AMPK subunit beta-2

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to AMPK beta 2

Concentration: 1mg/mL

Immunogen: Recombinant full length protein of human AMPK beta 2

WB description: Western blot analysis of AMPK beta 2 expression in MCF7 (A), mouse heart (B), rat heart (C) whole cell lysates.

IHC description: Immunohistochemical analysis of AMPK beta 2 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of AMPK beta 2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)

Gene ID(Human): 5565

Gene ID (Mouse): 108097

Gene ID(Rat): 64562

SwissProt (Human): O43741

SwissProt (Mouse): Q6PAM0

SwissProt (Rat): Q9QZH4

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.