GeneBio Systems
Purified Rabbit anti-AF4 Antibody
Purified Rabbit anti-AF4 Antibody
SKU:DL92270A
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Size 1: 100 ul
Synonyms : AF4; FEL; MLLT2; PBM1; AF4/FMR2 family member 1; ALL1-fused gene from chromosome 4 protein; Protein AF-4; Protein FEL; Proto-oncogene AF4
host: Rabbit
Reactivity(H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish): H, M, R
Applications: WB, IHC
Background: Rabbit polyclonal antibody to AF4
Concentration: 1mg/mL
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human AF4. The exact sequence is proprietary.
WB description: Western blot analysis of AF4 expression in HeLa (A), K562 (B), Jurkat (C) whole cell lysates.
IHC description: Immunohistochemical analysis of AF4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description:
IP description:
FC description:
ChIP description:
Purification_Method: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Conjugation:
Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene ID(Human): 4299
Gene ID (Mouse):
Gene ID(Rat):
SwissProt (Human): P51825
SwissProt (Mouse): O88573
SwissProt (Rat):
Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.
