Purified Rabbit anti-Adenylate Cyclase 9 Antibody

SKU: DL90020A

Size 1: 100 ul

Synonyms : KIAA0520; Adenylate cyclase type 9; ATP pyrophosphate-lyase 9; Adenylate cyclase type IX; Adenylyl cyclase 9

host: Rabbit

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC

Background: Rabbit polyclonal antibody to Adenylate Cyclase 9

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Adenylate Cyclase 9. The exact sequence is proprietary.

WB description: Western blot analysis of Adenylate Cyclase 9 expression in HeLa (A), SP2/0 (B), H9C2 (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Adenylate Cyclase 9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Adenylate Cyclase 9 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description:

Purification_Method: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene ID(Human): 115

Gene ID (Mouse): 11515

Gene ID(Rat):

SwissProt (Human): O60503

SwissProt (Mouse): P51830

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.