Purified Mouse anti-Histone H2B Antibody

SKU: DL95511A

Size 1: 100 ul

Synonyms : H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f

host: Mouse

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC

Background: Mouse monoclonal antibody to Histone H2B

Concentration: 1mg/mL

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Histone H2B. The exact sequence is proprietary.

WB description: Western blot analysis of Histone H2B expression in Hela (A), NIH3T3 (B), Raw264.7 (C), rat brain (D), rat kidney (E) whole cell lysates.

IHC description: Immunohistochemical analysis of Histone H2B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description:

Purification_Method:

Clonality: Monoclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/1000 - 1/3000), IH (1/200 - 1/500)

Gene ID(Human): 3018

Gene ID (Mouse): 319178

Gene ID(Rat):

SwissProt (Human): P33778

SwissProt (Mouse): Q64475

SwissProt (Rat):

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.