Purified Mouse anti-Alpha-tubulin Antibody

SKU: DL95446A

Size 1: 100 ul

Synonyms : TUBA3; Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain

host: Mouse

Reactivity（H- Human, M- Mouse, R- Rat, B- Bovine, C- Chicken, D- Dog, G- Goat, Mk- Monkey, P- Pig, Rb- Rabbit, S- Sheep, Z- Zebrafish）: H, M, R

Applications: WB, IHC, IF/IC, IP

Background: Mouse monoclonal antibody to Alpha-tubulin

Concentration: 1mg/mL

Immunogen: Recombinant protein corresponding to human Alpha-tubulin.

WB description: Western blot analysis of Alpha-tubulin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.

IHC description: Immunohistochemical analysis of Alpha-tubulin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description: Immunofluorescent analysis of Alpha-tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description: Immunoprecipitation of Alpha-tubulin from 0.5mg mouse brain whole cell extract lysate, using anti-Alpha-tubulin Antibody.

FC description:

ChIP description:

Purification_Method:

Clonality: Monoclonal

Conjugation:

Buffer: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% BSA, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/1000 - 1/3000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/50 - 1/100)

Gene ID(Human): 7846; 10376

Gene ID (Mouse): 22142; 22143

Gene ID(Rat): 64158; 500929

SwissProt (Human): Q71U36; P68363

SwissProt (Mouse): P68369; P05213

SwissProt (Rat): P68370; Q6P9V9

Storage: Store at -20℃. Avoid repeated freeze / thaw cycles.