Purified Anti-Human CD152 Antibody[9D9]

SKU: AN008120P

Size:1mg

Storage:Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Flow Cytometry Antibodies

Abbreviation:CD152

Target Synonym:GRD;Cytotoxic T-lymphocyte protein;Cytotoxic T-lymphocyte-associated antigen;IDDM;CELIAC;CELIAC3;CTLA-4;Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4;GRD4;GSE;IDDM12;Cd152;Ctla4; Ly-56;Ctla4;Ly-56

Research Areas:

Conjugation:Unconjugated

Host:Mouse

Species reactivity:Human

Application:FCM

Usage:

Isotype:Mouse IgG2b, κ

Clonality:Monoclonal

Clone NO.:9D9

UNIProt ID:P09793

Background:CTLA-4 and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. CTLA-4 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CTLA-4 and CD28 are composed of a single Ig V‑like extracellular domain, a transmembrane domain and an intracellular domain. CTLA-4 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on Human chromosome 2. CTLA-4 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CTLA-4 transcripts have since been found in both Th1 and Th2, and CD4+ and CD8+ T cell clones. Whereas, CD28 expression is constitutive on the surfaces of 95% of CD4+ T cells and 50% of CD8+ T cells and is down regulated upon T cell activation, CTLA-4 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with 20-100-fold higher affinity than CD28. The physiological role of CTLA-4 in T cell costimulation is currently being studied.

Concentration:≥ 1 mg/mL

Control catalog number:

Immunogen:Recombinant Human CD152 protein

Buffer:PBS, pH 7.2. Contains 0.05% proclin 300.

Purification method:>98%, Protein A/G purified

Dilution:FCM 2 μg/mL(0.5×10⁶-1×10⁶ cells)

GeneID:12477