Phospho-WEE1 (Ser642) Antibody - Cat. #: CSB-PA062918

SKU: CSB-PA062918

Size :100ul

Clone Number:

Aliases:DKFZp686I18166 antibody; EC 2.7.10.2 antibody; FLJ16446 antibody; MGC105683 antibody; OTTHUMP00000231338 antibody; OTTHUMP00000231339 antibody; Wee 1 antibody; WEE 1 homolog 1 (S. pombe) antibody; WEE1 antibody; WEE1 homolog (S. pombe) antibody; Wee1 homolog antibody; WEE1 homolog S. pombe antibody; Wee1 like protein kinase antibody; Wee1 tyrosine kinase antibody; Wee1+ homolog antibody; Wee1+ S. pombe homolog antibody; WEE1, S. pombe, homolog of antibody; WEE1, somatic antibody; Wee1-like protein kinase antibody; WEE1_HUMAN antibody; WEE1A antibody; Wee1A kinase antibody; WEE1hu antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:P30291

Immunogen:Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1.

Raised in:Rabbit

Species Reactivity:Human, Mouse, Rat

Tested Applications:ELISA, WB; WB:1:500-1:1000

Background:

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Watanabe N., EMBO J. 14:1878-1891(1995).
Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001).
Igarashi M., Nature 353:80-83(1991)

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Conjugate:Non-conjugated

Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:WEE1

Research Areas:Epigenetics and Nuclear Signaling;Cell biology