Phospho-VCL (Tyr822) Antibody - Cat. #: CSB-PA177557

SKU: CSB-PA177557

Size :100ul

Clone Number:

Aliases:CMD1W antibody; CMH15 antibody; Epididymis luminal protein 114 antibody; HEL114 antibody; Metavinculin antibody; MV antibody; MVCL antibody; OTTHUMP00000019861 antibody; OTTHUMP00000019862 antibody; VCL antibody; VINC antibody; VINC_HUMAN antibody; Vinculin antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:P18206

Immunogen:Peptide sequence around phosphorylation site of Tyrosine 822 (S-G-Y(p)-R-I) derived from Human Vinculin.

Raised in:Rabbit

Species Reactivity:Human, Mouse, Rat

Tested Applications:ELISA, WB; WB:1:500-1:1000

Background:

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Subauste MC, et al. (2004) J Cell Biol 165, 371-81
Olsen JV, et al. (2010) Sci Signal 3, ra3
J?rgensen C, et al. (2009) Science 326, 1502-9

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Conjugate:Non-conjugated

Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:VCL

Research Areas:Isotype/Loading Controls;Cardiovascular;Signal transduction