Phospho-Pim1 (Tyr309) Antibody - Cat. #: CSB-PA174999

SKU: CSB-PA174999

Size :100ul

Clone Number:

Aliases:Pim1 antibody; Pim-1 antibody; Serine/threonine-protein kinase pim-1 antibody; EC 2.7.11.1 antibody

Product Type:Polyclonal Antibody

Immunogen Species:Mus musculus (Mouse)

UniProt ID:P06803

Immunogen:Peptide sequence around phosphorylation site of tyrosine 309(A-A-V(p)-W-S) derived from Human Pim-1.

Raised in:Rabbit

Species Reactivity:Human, Mouse, Rat

Tested Applications:ELISA, WB; WB:1:500-1:1000

Background:

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis.

Selten G., Cell 46:603-611(1986).
Saris C.J., EMBO J. 10:655-664(1991).
Maita H., Eur. J. Biochem. 267:5168-5178(2000).

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Conjugate:Non-conjugated

Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:Pim1

Research Areas:Epigenetics and Nuclear Signaling;Cancer;Signal transduction?Stem cells