Phospho-NF-kB p65 (Ser536) Antibody , Cat. # AF2006

SKU: AF2006

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:RELA

GeneID:5970

Uniprot:Q04206

Specificity:Phospho-NF-kB p65 (Ser536) Antibody detects endogenous levels of NF-kB p65 only when phosphorylated at Serine 536.

Immunogen:A synthesized peptide derived from human NF- kappaB p65 around the phosphorylation site of Ser536.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, sodium azide and glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat,Monkey

Prediction:Pig,Bovine,Horse,Sheep,Dog

Application:WB,IHC,IF/ICC,IP,ELISA(peptide)

Dilution:WB 1:500-1:2000, IHC 1:50-1:500, IP 1:100-1:500, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000

Conjugate:Unconjugated

RRID:AB_2834435

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF2006-BP

Des# Blocking Peptide:Phospho-NF-kB p65 (Ser536) Blocking peptide

Blocking Peptide Price:$549 CAD