Gene Bio Systems
Phospho-GSK3 alpha/ beta (Tyr216/Tyr279) Antibody , Cat. # AF3335
Phospho-GSK3 alpha/ beta (Tyr216/Tyr279) Antibody , Cat. # AF3335
SKU:AF3335
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Size:100ul
Host:Rabbit
Clonality:Polyclonal
Isotype:IgG
GeneName:GSK3B,GSK3A
GeneID:2932,2931
Uniprot:P49840/P49841
Specificity:Phospho-GSK3 alpha/ beta (Tyr216/Tyr279) Antibody detects endogenous levels of GSK3 alpha/ beta only when phosphorylated at Tyrosine 279/216.
Immunogen:A synthesized peptide derived from human GSK3 alpha/ beta around the phosphorylation site of Tyr279/216.
Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity:Human,Mouse,Rat
Prediction:Pig,Bovine,Chicken,Xenopus
Application:WB,IHC,IF/ICC,ELISA(peptide)
Dilution:WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
Conjugate:Unconjugated
RRID:AB_2834750
An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide:AF3335-BP
Des# Blocking Peptide:Phospho-GSK3 alpha/ beta (Tyr216/Tyr279) Blocking peptide
Blocking Peptide Price:$549 CAD
