Phospho-EIF2S1 (Ser51) Antibody - Cat. #: CSB-PA285504

SKU: CSB-PA285504

Size :100ul

Clone Number:

Aliases:EIF 2 alpha antibody; EIF 2 antibody; EIF 2A antibody; EIF 2alpha antibody; eIF-2-alpha antibody; eIF-2A antibody; EIF-2alpha antibody; EIF2 alpha antibody; EIF2 antibody; EIF2A antibody; EIF2S1 antibody; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody; Eukaryotic translation initiation factor 2 subunit 1 alpha antibody; Eukaryotic translation initiation factor 2 subunit 1 antibody; Eukaryotic translation initiation factor 2 subunit alpha antibody; IF2A_HUMAN antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:P05198

Immunogen:Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2a.

Raised in:Rabbit

Species Reactivity:Human, Mouse, Rat

Tested Applications:ELISA, WB, IHC, IF; WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Background:

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Xavier Saelens, et al. (2001) J. Biol. Chem; 276: 41620 - 41628.
Hiroyuki Kubota, et al. (2003) J. Biol. Chem ; 278: 20457 - 20460.
Shijian Chu, et al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983 - L992.
Eileen Connolly, et al. (2006) Mol. Cell. Biol ; 26: 3955 - 3965.

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Conjugate:Non-conjugated

Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:EIF2S1

Research Areas:Epigenetics and Nuclear Signaling