Phospho-CCNB1 (Ser147) Antibody - Cat. #: CSB-PA059325

SKU: CSB-PA059325

Size :100ul

Clone Number:

Aliases:CCNB 1 antibody; CCNB antibody; ccnb1 antibody; CCNB1_HUMAN antibody; Cyclin B1 antibody; G2 mitotic specific cyclin B1 antibody; G2/mitotic-specific cyclin-B1 antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:P14635

Immunogen:Peptide sequence around phosphorylation site of Serine 147 (A-F-S(p)-D-V) derived from Human Cyclin B1.

Raised in:Rabbit

Species Reactivity:Human

Tested Applications:ELISA, WB, IHC, IF; WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200

Background:The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470.
Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685.
Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.

Clonality:Polyclonal

Isotype:IgG

Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Conjugate:Non-conjugated

Buffer:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:CCNB1

Research Areas:Epigenetics and Nuclear Signaling;Cancer;Cell biology