Phospho-4E-BP1 (Ser65/Thr70) Antibody , Cat. # AF2308

SKU: AF2308

Size:100ul

Host:Rabbit

Clonality:Polyclonal

Isotype:IgG

GeneName:EIF4EBP1

GeneID:1978

Uniprot:Q13541

Specificity:Phospho-4E-BP1 (Ser65/Thr70) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Ser65/Thr70.

Immunogen:A synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Ser65/Thr70.

Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Reactivity:Human,Mouse,Rat

Prediction:Pig,Bovine,Horse,Sheep,Dog,Chicken

Application:WB,IHC,IF/ICC

Dilution:WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Conjugate:Unconjugated

RRID:AB_2845322

An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.

Cat# Blocking Peptide:AF2308-BP

Des# Blocking Peptide:Phospho-4E-BP1 (Ser65/Thr70) Blocking peptide

Blocking Peptide Price:$549 CAD