PCNA Monoclonal Antibody

SKU: AN005350L

Size::25μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

Shipping:The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Tags/Controls Antibodies

Abbreviation:PCNA

Target Synonym:Proliferating cell nuclear antigen,PCNA,Cyclin

Research Areas:Cell Biology;Epigenetics and Nuclear Signaling;Tags & Cell Markers;Cancer;Isotype;Loading Controls

Conjugation:Unconjugated

Host:Mouse

Species reactivity:Human;Mouse;Rat

Application:WB;IP;IHC

Isotype:IgG2a

Clonality:Monoclonal

Clone NO.:9C6

UNIProt ID:P12004

Accession:

Background:Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Concentration:1 mg/mL

Immunogen:Recombinant human PCNA protein expressed by E.coli

Buffer:PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH 7.4

Purification method:Protein A/G Purification

Dilution:WB 1:2000-1:4000;IP 4ug/sample;IHC 1:200-1:400

Calculated MW:29 kDa

ObservedMW:33 kDa