One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594)

SKU: E-CK-A322

Size:100Assays

Storage:This product can be stored at -20°C for 12 months with shading light.

Shipping:Gel Packs

Exp date:12 months

Category ID_I:Apoptosis and Cell Health Detection

Category ID_II:Cell Apoptosis

Category ID_III:TUNEL Assay Kits

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Kit Type:Apoptosis/DNA Fragmentation

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Detection Instrument:Fluorescence Microscope

Tested Sample Types:Paraffin section;frozen section;cell slide

Assay Time:3 hours

Detection Type:Fluorometric method

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Test Principle:When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

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