Mouse LIN28A(Lin-28 Homolog A) ELISA Kit

SKU: ELK7935

Size:96T

Reactivity:Mouse

Alternative Names:ZCCHC1; CSDD1; LIN28; Zinc finger CCHC domain-containing protein 1

Assay Type:Sandwich

Sensitivity:0.049 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:Tissue homogenates and other biological fluids.

Assay length:3.5h

Research Area:Signal transduction;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LIN28A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LIN28A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LIN28A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LIN28A in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q8K3Y3