Mouse Kelch-like Ech-associated Protein 1, KEAP1 ELISA Kit

SKU: E2198Mo

Size:96 Tests-1 Kit

Background:Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination (PubMed: 9887101, PubMed: 12682069, PubMed: 15282312, PubMed: 15367669, PubMed: 15581590). KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes (PubMed: 9887101, PubMed: 12193649, PubMed: 14764894). In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes (PubMed: 12193649, PubMed: 20$498.00|$360.00371, PubMed: 22014577, PubMed: 29590092). In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2 (PubMed: 20421418, PubMed: 20173742, PubMed: 24011591). The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation (PubMed: 28380357). The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome (By similarity).

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:KEAP1

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:0.97ng/L

Detection range:2-600ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Kelch-Like Ech-Associated Protein 1

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9Z2X8

MASS(Da):69,553

GeneID:50868

Synonyms:Cytosolic inhibitor of Nrf2;INrf2;KEAP 1;KEAP1;Kelch-like ECH-associated protein 1

Gene names:KEAP1

Standard solution:640ng/L