Mouse Hyp(Hydroxyproline) ELISA Kit

SKU: ELK9115

Size:96T

Reactivity:Mouse

Alternative Names:(2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)

Assay Type:Competitive Inhibition

Sensitivity:27.3 ng/mL

Standard:5000 ng/mL

Detection range:78.13-5000 ng/mL

Sample type:serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Metabolic pathway;Nutrition metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Hyp. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Hyp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Hyp in the samples is then determined by comparing the OD of the samples to the standard curve.

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