Mouse Heat shock factor protein 1,Hsf1 ELISA KIT

SKU: E0176Mo

Size:96 Tests-1 Kit

Background:Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage. In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form. Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes. Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form. Binds to inverted 5'-NGAAN-3' pentamer DNA sequences. Binds to chromatin at heat shock gene promoters. Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells. Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner. Plays a role in nuclear export of stress-induced HSP70 mRNA. Plays a role in the regulation of mitotic progression. Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner. Involved in stress-induced cancer cell proliferation in a IER5-dependent manner. {ECO:0000250|UniProtKB:Q00613}.

Research_area:

Applications:ELISA

Target_protein:Hsf1

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:0.67ng/ml

Detection range:1.25-80ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Heat shock factor protein 1

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P38532

MASS(Da):57223

GeneID:15499

Synonyms:Hsf1

Gene names:Hsf1

Standard solution:160ng/ml