Mouse GIP(Gastric Inhibitory Polypeptide) ELISA Kit

SKU: ELK1582

Size:96T

Reactivity:Mouse

Alternative Names:Incretin hormone; Glucose Dependent Insulinotropic Peptide

Assay Type:Competitive Inhibition

Sensitivity:24.5 pg/mL

Standard:5000 pg/mL

Detection range:78.13-5000 pg/mL

Sample type:Serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay length:2h

Research Area:Endocrinology;Gastroenterology;Hormone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse GIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GIP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P48756