Mouse Epidermal Growth Factor Receptor, EGFR ELISA Kit

SKU: E0993Mo

Size:96 Tests-1 Kit

Background:Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed: 8404850). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (By similarity). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (By similarity). Plays a role in enhancing learning and memory performance (PubMed: 20639532).

Research_area:Signal Transduction

Applications:ELISA

Target_protein:EGFR

Reactivity:Mouse

Assay type:Sandwich

Sensitivity:0.24ng/mL

Detection range:0.5-100ng/mL

Sample type:Serum, plasma or other biological fluids

Full product name:Epidermal Growth Factor Receptor

Species:Mouse

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q01279

MASS(Da):134,853

GeneID:13649

Synonyms:EGFR;Epidermal growth factor receptor

Gene names:EGFR

Standard solution:128ng/mL