MAPKAPK3 Antibody - Cat. #: CSB-PA247267

SKU: CSB-PA247267

Size :100ul

Clone Number:

Aliases:3pK antibody; anti MAPKAPK3 antibody; Chromosome 3p kinase antibody; EC 2.7.11.1 antibody; MAP kinase-activated protein kinase 3 antibody; MAPK activated protein kinase 3 antibody; MAPK-activated protein kinase 3 antibody; MAPK3_HUMAN antibody; MAPKAP antibody; MAPKAP kinase 3 antibody; MAPKAP-K3 antibody; MAPKAP3 antibody; MAPKAPK 3 antibody; MAPKAPK-3 antibody; Mapkapk3 antibody; MAPKAPK3 antibody antibody; Mitogen activated protein kinase activated protein kinase 3 antibody; Mitogen activated protein kinase activated protein kinase antibody; MK-3 antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:Q16644

Immunogen:Synthesized peptide derived from internal of Human MAPK3.

Raised in:Rabbit

Species Reactivity:Human, Mouse, Rat

Tested Applications:ELISA, WB, IHC; WB:1:500-1:3000, IHC:1:50-1:100

Background:Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.

McLaughlin M.M., J. Biol. Chem. 271:8488-8492(1996).
Sithanandam G., Mol. Cell. Biol. 16:868-876(1996).
Neufeld B., J. Biol. Chem. 275:20239-20242(2000).

Clonality:Polyclonal

Isotype:IgG

Purification Method:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugate:Non-conjugated

Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form:liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:MAPKAPK3

Research Areas:Signal transduction