Human Ubiquitin-like-conjugating Enzyme Atg3, ATG3 ELISA Kit

SKU: E7300Hu

Size:96 Tests-1 Kit

Background:E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Research_area:Cell Biology

Applications:ELISA

Target_protein:ATG3

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.015ng/ml

Detection range:0.05-15ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Ubiquitin-Like-Conjugating Enzyme ATG3

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9NT62

MASS(Da):35,864

GeneID:64422

Synonyms:APG3-like;ATG 3;ATG3;Autophagy-related protein 3;hApg3;Protein PC3-96;Ubiquitin-like-conjugating enzyme ATG3

Gene names:ATG3

Standard solution:16ng/ml