Human UBE3A(Ubiquitin Protein Ligase E3A) ELISA Kit

SKU: ELK1751

Size:96T

Reactivity:Human

Alternative Names:ANCR; AS; E6-AP; EPVE6AP; HPVE6A; Papilloma Virus E6-Associated,Angelman Syndrome; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54

Assay Type:Sandwich

Sensitivity:0.122 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Enzyme & Kinase;Developmental science;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UBE3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UBE3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UBE3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UBE3A in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q05086