Human TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

SKU: ELK9590

Size:96T

Reactivity:Human

Alternative Names:ACP5;TRAP5b;SPENCDI

Assay Type:Sandwich

Sensitivity:0.45 ng/mL

Standard:50 ng/mL

Detection range:0.78-50 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Signal Transduction;Signal Transduction;Metabolism

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.

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