Human TPI1(Triosephosphate Isomerase 1) ELISA Kit

SKU: ELK3279

Size:96T

Reactivity:Human

Alternative Names:TIM; Triose-phosphate isomerase

Assay Type:Sandwich

Sensitivity:0.242 ng/mL

Standard:40 ng/mL

Detection range:0.63-40 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:3.5h

Research Area:Enzyme & Kinase;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPI1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P60174