Human Sonic hedgehog,SHH ELISA KIT

SKU: E6203Hu

Size:96 Tests-1 Kit

Background:[Sonic hedgehog protein]: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum (By similarity). {ECO:0000250|UniProtKB:Q62226}.; [Sonic hedgehog protein N-product]: The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites (PubMed:24863049). Involved in the patterning of the anterior-posterior axis of the developing limb bud (By similarity). Essential for axon guidance (By similarity). Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PubMed:10753901). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (PubMed:10753901). {ECO:0000250|UniProtKB:Q62226, ECO:0000269|PubMed:10753901, ECO:0000269|PubMed:24863049, ECO:0000303|PubMed:24522195}.

Research_area:

Applications:ELISA

Target_protein:SHH

Reactivity:Human

Assay type:Sandwich

Sensitivity:9.92ng/L

Detection range:20-7000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Sonic hedgehog

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q15465

MASS(Da):49607

GeneID:6469

Synonyms:SHH

Gene names:SHH

Standard solution:8000ng/L