Human Serine Threonine-protein Kinase Endoribonuclease Ire1, ERN1 ELISA Kit

SKU: E6150Hu

Size:96 Tests-1 Kit

Background:Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875, PubMed:28128204). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed:21884936, PubMed:28067262).

Research_area:Signal Transduction

Applications:ELISA

Target_protein:ERN1

Reactivity:Human

Assay type:Sandwich

Sensitivity:7.76ng/L

Detection range:15-3000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Serine/threonine-protein kinase/endoribonuclease IRE1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:O75460

MASS(Da):109,735

GeneID:2081

Synonyms:Endoplasmic reticulum-to-nucleus signaling 1;Endoribonuclease;ERN1;hIRE1p;Inositol-requiring protein 1;IRE1;IRE1a;IRE1a [Includes: Serine/threonine-protein kinase;Ire1-alpha;IRE1P;Serine/threonine-protein kinase/endoribonuclease IRE1

Gene names:ERN1

Standard solution:3200ng/L