Human Prosaposin, PSAP ELISA Kit

SKU: E5938Hu

Size:96 Tests-1 Kit

Background:Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate. Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases. Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12). [Prosaposin]: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling. Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.

Research_area:Metabolism

Applications:ELISA

Target_protein:PSAP

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.054ng/mL

Detection range:0.1-40ng/mL

Sample type:Serum, plasma or other biological fluids

Full product name:Prosaposin

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P07602

MASS(Da):58,113

GeneID:5660

Synonyms:A1 activator;Cerebroside sulfate activator;Co-beta-glucosidase;Component C;CSAct;Dispersin;Glucosylceramidase activator;Proactivator polypeptide;Prosaposin;Protein A;Protein C;PSAP;SAP-1;SAP-2;Saposin-A;Saposin-B;Saposin-B-Val;Saposin-C;Saposin-D;Sphingolipid activator protein 1;Sphingolipid activator protein 2;Sulfatide/GM1 activator;

Gene names:PSAP

Standard solution:48ng/mL