Human Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase,NGLY1 ELISA KIT

SKU: E5839Hu

Size:96 Tests-1 Kit

Background:Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. {ECO:0000269|PubMed:14749736, ECO:0000269|PubMed:15358861}.

Research_area:

Applications:ELISA

Target_protein:NGLY1

Reactivity:Human

Assay type:Sandwich

Sensitivity:9.12ng/L

Detection range:20-4500ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q96IV0

MASS(Da):74390

GeneID:55768

Synonyms:NGLY1 PNG1

Gene names:NGLY1

Standard solution:4800ng/L