Human OPN/Osteopontin EZ-Set™ ELISA Kit (DIY Antibody Pairs)

SKU: EZ0482

Size: 5 plates/kit

Storage: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Form:

Reactivity: Human

Applications: ELISA

Application Details:

Gene Name: SPP1

Specificity: Natural and recombinant human OPN

Background: Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.

Immunogen: Expression system for standard: NS0; Immunogen sequence: I17-N300

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Reference:

Uniprot ID: P10451

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Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

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Gene ID: 6696

Protein Name: Osteopontin

Gene Full Name: secreted phosphoprotein 1

Synonyms: BNSP; Bone sialoprotein 1; BSPI; ETA 1; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1; SPP 1; SPP1; Urinary stone protein; Uropontin