Human M-phase Phosphoprotein 8, MPHOSPH8 ELISA Kit

SKU: E2915Hu

Size:96 Tests-1 Kit

Background:Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed: 20871592, PubMed: 26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed: 26022416, PubMed: 28581500). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed: 20871592). Mediates down-regulation of CDH1 expression (PubMed: 20871592). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed: 29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed: 30487602).

Research_area:Others

Applications:ELISA

Target_protein:MPHOSPH8

Reactivity:Human

Assay type:Sandwich

Sensitivity:1.17ng/L

Detection range:2-700ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:M-Phase Phosphoprotein 8

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q99549

MASS(Da):97,182

GeneID:54737

Synonyms:M-phase phosphoprotein 8;MPHOSPH 8;MPHOSPH8;Twa3;Two hybrid-associated protein 3 with RanBPM

Gene names:MPHOSPH8

Standard solution:800ng/L