Human Lysine-specific demethylase 5C (KDM5C) ELISA Kit

SKU: AE37016HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: Q38JA7

Abbreviation: KDM5C

Alternative Names: RP11-258C19.2; DXS1272E; JARID1C; MRXJ; MRXSJ; SMCX; XE169; JmjC domain-containing protein SMCX|Jumonji; AT rich interactive domain 1C (RBP2-like)|Jumonji/ARID domain-containing protein 1C|Smcx homo

Application: ELISA

Range: 15.6-1000 pg/mL

Sensitivity: 8.2 pg/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate KDM5C in samples. An antibody specific for KDM5C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKDM5C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KDM5C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KDM5C bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: KDM5C is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. The DNA-binding motifs suggest this protein is involved in the regulation of transcription and chromatin remodeling. Alternative splicing generates 2 distinct transcripts, either containing or missing 9 nucleotides, which in turn predicts 2 XE169 protein isoforms composed of 1,557 and 1,560 amino acids, respectively. XE169 is expressed in multiple human tissues, and homologous sequences exist on the human Y chromosome and in the genomes of 5 other eutherian mammals examined. RT-PCR analysis of somatic cell hybrids containing either an active or an inactive human X chromosome on a rodent background demonstrated that XE169 escapes X inactivation.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).