Human Interleukin-1 Receptor-associated Kinase 4, IRAK-4 ELISA Kit

SKU: E5506Hu

Size:96 Tests-1 Kit

Background:Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed: 17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

Research_area:Immunology

Applications:ELISA

Target_protein:IRAK4

Reactivity:Human

Assay type:Sandwich

Sensitivity:1.31ng/L

Detection range:3-900ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Interleukin-1 Receptor-Associated Kinase 4

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q9NWZ3

MASS(Da):51,530

GeneID:51135

Synonyms:Interleukin-1 receptor-associated kinase 4;IRAK 4;IRAK4;IRAK-4;Renal carcinoma antigen NY-REN-64

Gene names:IRAK4

Standard solution:960ng/L