Human Interferon-induced 35 kDa Protein, IFI35 ELISA Kit

SKU: E5498Hu

Size:96 Tests-1 Kit

Background:Acts as a signaling pathway regulator involved in innate immune system response (PubMed: 26342464, PubMed: 29038465, PubMed: 29350881). In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation (PubMed: 10779520, PubMed: 10950963). In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production (PubMed: 26342464). In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (PubMed: 29350881). Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion (PubMed: 29038465). Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines (PubMed: 29038465).

Research_area:Cell Biology

Applications:ELISA

Target_protein:IFI35

Reactivity:Human

Assay type:Sandwich

Sensitivity:7.28ng/L

Detection range:15-3000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Interferon-Induced 35 Kda Protein

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P80217

MASS(Da):31,546

GeneID:3430

Synonyms:IFI 35;IFI35;Ifi-35;IFP 35;Interferon-induced 35 kDa protein

Gene names:IFI35

Standard solution:3200ng/L