Human Hypoxia-inducible Factor 1Α, HIF-1A ELISA Kit

SKU: E0422Hu

Size:96 Tests-1 Kit

Background:Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, is required for induction of glycolysis in monocytes and the consequent proinflammatory state (PubMed: 32697943). In monocytes, induces expression of ACE2 and cytokines such as IL1B, TNF, IL6, and interferons (PubMed: 32697943). Promotes human coronavirus SARS-CoV-2 replication and monocyte inflammatory response (PubMed: 32697943).

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:HIF1A

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.01ng/ml

Detection range:0.05-15ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Hypoxia-Inducible Factor 1 alpha

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q16665

MASS(Da):92,670

GeneID:3091

Synonyms:ARNT-interacting protein;Basic-helix-loop-helix-PAS protein MOP1;bHLHe78;Class E basic helix-loop-helix protein 78;HIF 1A;HIF1A;HIF1-alpha;HIF-1-alpha;Hypoxia-inducible factor 1-alpha;Member of PAS protein 1;PAS domain-containing protein 8

Gene names:HIF1A

Standard solution:16ng/ml