Human Histone-lysine N-methyltransferase Ezh2, EZH2 ELISA Kit

SKU: E5422Hu

Size:96 Tests-1 Kit

Background:Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed:22323599, PubMed:30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.

Research_area:Others

Applications:ELISA

Target_protein:EZH2

Reactivity:Human

Assay type:Sandwich

Sensitivity:9.93ng/L

Detection range:20-4500ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Histone-lysine N-methyltransferase EZH2

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q15910

MASS(Da):85,363

GeneID:2146

Synonyms:Enhancer of zeste homolog 2;ENX1;ENX-1;EZH2;EZH2b;Histone-lysine N-methyltransferase EZH2;KMT6;Lysine N-methyltransferase 6;WVS;WVS2

Gene names:EZH2

Standard solution:4800ng/L