Human Duffy antigen/chemokine receptor (DARC) ELISA Kit

SKU: AE58935HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: Q16570

Abbreviation: DARC

Alternative Names: CTA-134P22.3; CCBP1; CD234; Dfy; FY; GPD; GpFy; WBCQ1; Duffy blood group antigen|Fy glycoprotein|OTTHUMP00000035293|OTTHUMP00000035294|glycoprotein D

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.062 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate DARC in samples. An antibody specific for DARC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDARC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DARC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DARC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Duffy blood group, chemokine receptor (DARC) acts as a non-classical chemokine receptor that does not signal but rather acts as a scavenger protein. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.The Duffy system enjoys the distinction of being the first blood group whose genetic locus was assigned to a specific autosome, i.e., chromosome 1. On the basis of families studied in Rochester, N.Y., Weitkamp (1972) could demonstrate no linkage of the HBB locus and Duffy, as had been suggested by Nance et al. (1970). An earlier suspicion of localization to chromosome 16 was apparently in error. Duffy and the locus for a form of hereditary cataract are closely linked. From extensive family studies, Robson et al. (1973) arrived at a tentative map of chromosome 1.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).