Human Cytokeratin 20 (CK20) ELISA Kit

SKU: AE36338HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: P35900

Abbreviation: KRT20

Alternative Names: CD20; CK20; K20; KRT21; MGC35423; cytokeratin 20|keratin; type I cytoskeletal 20

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.063 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate KRT20 in samples. An antibody specific for KRT20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Cytokeratin 20(CK20) is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract. Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium. CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).