Human Cytochrome P450 2C9, CYP2C9 ELISA Kit

SKU: E5160Hu

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and steroids (PubMed: 7574697, PubMed: 9866708, PubMed: 9435160, PubMed: 12865317, PubMed: 15766564, PubMed: 19965576, PubMed: 21576599). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed: 7574697, PubMed: 9866708, PubMed: 9435160, PubMed: 12865317, PubMed: 15766564, PubMed: 19965576, PubMed: 21576599). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA) (PubMed: 7574697, PubMed: 15766564, PubMed: 19965576, PubMed: 9866708). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed: 21576599). Exhibits low catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2 (PubMed: 12865317). Catalyzes bisallylic hydroxylation and hydroxylation with double-bond migration of polyunsaturated fatty acids (PUFA) (PubMed: 9866708, PubMed: 9435160). Also metabolizes plant monoterpenes such as limonene. Oxygenates (R)- and (S)-limonene to produce carveol and perillyl alcohol (PubMed: 11950794). Contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan (PubMed: 25994031).

Research_area:Cardiovascular

Applications:ELISA

Target_protein:CYP2C9

Reactivity:Human

Assay type:Sandwich

Sensitivity:7.59ng/L

Detection range:15-3000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Cytochrome P450 2C9

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P11712

MASS(Da):55,628

GeneID:1559

Synonyms:(R-limonene 6-monooxygenase;(S-limonene 6-monooxygenase;(S-limonene 7-monooxygenase;Cholesterol 25-hydroxylase;CYP2C9;CYPIIC9;Cytochrome P450 2C9;Cytochrome P450 MP-4;Cytochrome P450 MP-8;Cytochrome P450 PB-1;Cytochrome P-450MP;S-mephenytoin 4-hydroxylase

Gene names:CYP2C9

Standard solution:3200ng/L