Human Cytochrome P450 1A2, CYP1A2 ELISA Kit

SKU: E2281Hu

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed: 9435160, PubMed: 10681376, PubMed: 11555828, PubMed: 12865317, PubMed: 19965576). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed: 9435160, PubMed: 10681376, PubMed: 11555828, PubMed: 12865317, PubMed: 19965576). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed: 11555828, PubMed: 12865317). Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2 (PubMed: 11555828, PubMed: 12865317). Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed: 21576599). May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed: 10681376). Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer (PubMed: 19965576). Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA (PubMed: 9435160). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed: 21068195). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin (PubMed: 14725854). Metabolizes caffeine via N3-demethylation (Probable).

Research_area:Others

Applications:ELISA

Target_protein:CYP1A2

Reactivity:Human

Assay type:Sandwich

Sensitivity:5.15ng/L

Detection range:10-2000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Cytochrome P450 1A2

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P05177

MASS(Da):58,407

GeneID:1544

Synonyms:Cholesterol 25-hydroxylase;CYP1A2;CYPIA2;Cytochrome P(3450;Cytochrome P450 1A2;Cytochrome P450 4;Cytochrome P450-P3;Hydroperoxy icosatetraenoate dehydratase

Gene names:CYP1A2

Standard solution:2400ng/L