Human Cytochrome P450 1A1, CYP1A1 ELISA Kit

SKU: E3222Hu

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed: 11555828, PubMed: 14559847, PubMed: 12865317, PubMed: 15805301, PubMed: 15041462, PubMed: 18577768, PubMed: 19965576, PubMed: 20972997, PubMed: 10681376). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed: 11555828, PubMed: 14559847, PubMed: 12865317, PubMed: 15805301, PubMed: 15041462, PubMed: 18577768, PubMed: 19965576, PubMed: 20972997, PubMed: 10681376). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions (PubMed: 11555828, PubMed: 14559847, PubMed: 12865317, PubMed: 15805301). Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (PubMed: 15041462, PubMed: 18577768). Catalyzes the epoxidation of double bonds of certain PUFA (PubMed: 15041462, PubMed: 19965576, PubMed: 20972997). Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system (PubMed: 20972997). Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (PubMed: 15041462). May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed: 10681376). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed: 21068195).

Research_area:Cardiovascular

Applications:ELISA

Target_protein:CYP1A1

Reactivity:Human

Assay type:Sandwich

Sensitivity:5.25ng/L

Detection range:10-2000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Cytochrome P450 1A1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P04798

MASS(Da):58,165

GeneID:1543

Synonyms:CYP 1A1;CYP1A1;CYPIA1;Cytochrome P450 1A1;Cytochrome P450 form 6;Cytochrome P450-C;Cytochrome P450-P1;Hydroperoxy icosatetraenoate dehydratase

Gene names:CYP1A1

Standard solution:2400ng/L