Human Cholesterol Side-chain Cleavage Enzyme, CYP11A1 ELISA Kit

SKU: E6517Hu

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed:21636783). Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed:21636783). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:21636783).

Research_area:Cardiovascular; Signal Transduction; Cancer; Metabolism

Applications:ELISA

Target_protein:CYP11A1

Reactivity:Human

Assay type:Sandwich

Sensitivity:8.07ng/L

Detection range:15-3000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Cholesterol side-chain cleavage enzyme

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P05108

MASS(Da):60,102

GeneID:1583

Synonyms:Cholesterol desmolase;Cholesterol side-chain cleavage enzyme, mitochondrial;CYP11A;CYP11A1;CYPXIA1;Cytochrome P450 11A1;Cytochrome P450(scc;P450SCC

Gene names:CYP11A1

Standard solution:3200ng/L