Human Centromere-associated Protein E, CENPE ELISA Kit

SKU: E2885Hu

Size:96 Tests-1 Kit

Background:Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules (PubMed:7889940, PubMed:23891108, PubMed:25395579). The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated (PubMed:25908662). Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends (PubMed:23955301). Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized (PubMed:25743205). Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules (PubMed:17535814) The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss (By similarity).

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:CENPE

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.049ng/ml

Detection range:0.1-35ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Centromere-associated protein E

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q02224

MASS(Da):316,415

GeneID:1062

Synonyms:CENPE;CENP-E;Centromere protein E;Centromere-associated protein E;KIF10;Kinesin-7;Kinesin-related protein CENPE;MCPH13;PPP1R61;

Gene names:CENPE

Standard solution:40ng/ml