Human CD209(CD209 Antigen) Microsample ELISA Kit

SKU: ELK0109MS

Size:96T

Reactivity:Human

Alternative Names:C-type lectin domain family 4 member L; Dendritic cell-specific ICAM-3-grabbing non-integrin 1; DC-SIGN; DC-SIGN1

Assay Type:Sandwich

Sensitivity:25.9 pg/mL

Standard:5000 pg/mL

Detection range:78.13-5000 pg/mL

Sample type:Serum, plasma, tissue homogenates

Assay length:3.5h

Research Area:CD & Adhesion molecule;Tumor immunity;Reproductive science;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD209. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD209. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD209, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD209 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q9NNX6