Human CA19-9(Carbohydrate Antigen 19-9) Microsample ELISA Kit

SKU: ELK1198MS

Size:96T

Reactivity:Human

Alternative Names:sialylated Lewis(a)Antigen; Cancer Antigen 19-9

Assay Type:Sandwich

Sensitivity:0.28 U/mL

Standard:50 U/mL

Detection range:0.79-50 U/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length:3.5h

Research Area:Tumor immunity;Hepatology;Gastroenterology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

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