Human Bromodomain Testis-specific Protein, BRDT ELISA Kit

SKU: E3880Hu

Size:96 Tests-1 Kit

Background:Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed: 22464331, PubMed: 22901802). Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed: 22901802). In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed: 22901802). Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (By similarity). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (By similarity). Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.

Research_area:Epigenetics and Nuclear Signaling

Applications:ELISA

Target_protein:BRDT

Reactivity:Human

Assay type:Sandwich

Sensitivity:13.85ng/L

Detection range:30-9000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Bromodomain Testis-Specific Protein

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q58F21

MASS(Da):107,954

GeneID:676

Synonyms:BRDT;Bromodomain testis-specific protein;Cancer/testis antigen 9;CT9;RING3-like protein

Gene names:BRDT

Standard solution:9600ng/L